Identifying novel proteins recognizing histone modifications using peptide pull-down assay
نویسندگان
چکیده
منابع مشابه
In vitro pull-down assay without expression constructs.
The in vitro pull-down assay is a well-established method to confirm direct binding in protein-protein interactions that was inferred from other interaction assays, such as two-hybrid analysis (1). The assay is usually carried out using glutathione-S-transferasetagged or His-tagged fusion proteins as the pull-down drivers and in vitro-translated 35S-labeled proteins as probes to detect interact...
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Alpha-synuclein (α-SYN) is a central molecule in Parkinson’s disease pathogenesis. Despite several studies, the molecular nature of endogenous α-SYN especially in human brain samples is still not well understood due to the lack of reliable methods and the limited amount of bio-specimens. Here, we introduce α-SYN single-molecule pull-down (α-SYN SiMPull) assay combined with in vivo protein cross...
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The identification and quantitative analysis of protein-protein interactions are essential to the functional characterization of proteins in the post-proteomics era. The methods currently available are generally time-consuming, technically complicated, insensitive and/or semi-quantitative. The lack of simple, sensitive approaches to precisely quantify protein-protein interactions still prevents...
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ژورنال
عنوان ژورنال: Methods
سال: 2006
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2006.05.028